The hydroxy fatty acids: Isolation, structure determination, quantitation

Isolation and determination methods for brain cerebrosides, hydroxy fatty acids, and unsaturated and saturated fatty acids *

A new method is described for the isolation of nearly pure total cerebrosides from fresh brain. Florisil columns are used to separate the cerebroside hydroxy and normal acids, in the form of their esters, and to separate the saturated and unsaturated esters of each group. The esters within each class are determined by gas chromatography, the hydroxy esters being run as the methyl ethers. Yields...

Determination of Tocopherols and Fatty Acids in Seeds of

  Background: The dried fruits of Silybum marianum (L.) Gaertn (Compositae) contain silymarin, an isomeric mixture of flavonolignans. Silymarin acts as a strong anti -hepatotoxic. This fruits contains a high amount of oil .  Objective: The objective of this study was to determine total lipid content, fatty acid composition and content of different kind of tocopherols in seed oils of S. marianum...

Cryptococcal 3-Hydroxy Fatty Acids Protect Cells Against Amoebal Phagocytosis

We previously reported on a 3-hydroxy fatty acid that is secreted via cryptococcal capsular protuberances - possibly to promote pathogenesis and survival. Thus, we investigated the role of this molecule in mediating the fate of Cryptococcus (C.) neoformans and the related species C. gattii when predated upon by amoebae. We show that this molecule protects cells against the phagocytic effects of...

Occurrence of 2-hydroxy Fatty Acids in Animal Tissues.

Mono (S) hydroxy fatty acids: novel ligands for cytosolic actin.

The ubiquitous hydroxylated fatty acids derived from arachidonic acid (HETEs) or linoleic acid (HODEs) exhibit diverse biological effects including chemotaxis, cell proliferation, and modulation of several enzymatic pathways, including the 5-lipoxygenase leading to the inflammatory leukotrienes. It was observed that 12(S)- and 15(S)-HETE and 13(S)-HODE (12- and 15-lipoxygenase-derived metabolit...